RBS
Spacer+RBS

Part:BBa_K3183020

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-16)


Operon Spacer for E. coli

This part is used for composite parts. It separated protein coding regions inside the operon and enables independent translation. A high-efficiency ribosome binding site is also located inside this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Use by Team Oxford 2019

This part was used inside our construct intended to attempt recombinant expression of AgrB and AgrD from C. difficile in E. coli. This part is BBa_K3183202.

References

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Categories
Parameters
None